PURINES, PYRIMIDINES, NUCLEOSIDE
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- DNA Stores Genetic Information.
- The transformation was inherited
permanently by subsequent generations.
- The early x-ray diffraction
results indicated that DNA was composed of two strands of the polymer wound
into a helix.
- The observation that DNA was
double-stranded was of crucial significance and provided one of the major clues
that led to the Watson-Crick structure of DNA.
Purines and pyrimidines
- The transformation was inherited permanently by subsequent generations.
- The early x-ray diffraction results indicated that DNA was composed of two strands of the polymer wound into a helix.
- The observation that DNA was double-stranded was of crucial significance and provided one of the major clues that led to the Watson-Crick structure of DNA.
- Purines and pyrimidines are nitrogen-containing heterocycles.
- cyclic compounds whose rings contain both carbon and other elements.
- They form Nucleosides and Nucleotides .
Nucleosides:
- these Are derivatives of purines and pyrimidines that have a sugar linked to a ring nitrogen.The sugar in ribonucleosides is D-ribose, and in deoxyribonucleosides it is 2-deoxy-D-ribose.
- ribonucleoside
- deoxyribonucleoside
- ribonucleosides
- deoxyribonucleoside
Structure of purine and pyrimidine nucleotides
- nucleotide = ester of phosphoric acid and a nucleoside
- nucleoside = N-containing base + monosaccharide
b-N-glycosidic bond between base and saccharide
almost always to N-1 of a pyrimidine or to N-9 of a purine
- Nucleotides are the building blocks of nucleic acids.
- Mononucleotides are nucleosides with a phosphoryl group esterified to a hydroxyl group of the sugar.
- Since most nucleotides are 5′-, the prefix “5′-” is usually omitted when naming them.
Functions of Nucleotides
- monomeric units of RNA and DNA substrates: nucleoside triphosphate
- energetic metabolismATP = principal form of chemical energy available to cells.
- physiological mediators ; cAMP, cGMP („second messengers“)
- components of coenzyme ; NAD+, NADP+, FAD
- allosteric efectors : regulation of key enzymes of metabolic pathways
SYNTHETIC NUCLEOTIDE ANALOGS ARE USED IN CHEMOTHERAPY
Nucleic Acid Structure & Function
Structure of DNA
- DNA Contains Four Deoxynucleotides
- Nucleotides have three characteristic components:
- nitrogenous (nitrogen-containing) base, pyrimidine and purine.
- pentose,
- phosphate.
- The molecule without the phosphate group is called a nucleoside. Both DNA and RNA contain two major purine bases,
- adenine (A) and
- guanine (G) Two major pyrimidines. In both DNA and RNA one of the pyrimidines is
- cytosine (C), but The second major pyrimidine is not the same in both: it is
- thymine (T) in DNA and
- uracil(U) in RNA.
- I.e; (A, G, C, T) in DNA , (A, G, C, U) in RNA
- These monomeric units of DNA are held in polymeric form by 3′,5′-phosphodiester bridges constituting a single strand
- The informational content of DNA (the genetic code) resides in the sequence in which these monomers are ordered.
- Together with x-ray data from the DNA molecule and the observation of Chargaff: That in DNA molecules the concentration of deoxyadenosine (A) nucleotides equals that of (T) nucleotides (A = T), while the concentration of (G) nucleotides equals that of (C) nucleotides (G = C), led Watson, Crick, and Wilkins to propose a model of a double stranded DNA molecule.
- The two strands of this double-stranded helix are held together by hydrogen bonds between the purine and pyrimidine bases.
- The pairings between the purine and pyrimidine nucleotides on the opposite strands are very specific; A with T and G with C .
- The two strands of the double-helical molecule, each of which possesses a polarity, are antiparallel; ie, one strand runs in the 5′ to 3′ direction and The other in the 3′ to 5′ direction.
- DNA PROVIDES A TEMPLATE FOR REPLICATION & TRANSCRIPTION
DNA functions :
- Store genetic information & transferring it to daughter cells during mitosis by replication
- Transfer of genetic information from DNA to RNA to be expressed as proteins
- THE CHEMICAL NATURE OF RNA DIFFERS FROM THAT OF DNA
- In RNA, the sugar moiety is ribose rather than the 2′-deoxyribose of DNA.
- The pyrimidine components of RNA differ from those of DNA. thymine (T) in DNA and uracil (U) in RNA.
- RNA exists as a single strand, whereas DNA exists as a double-stranded helical molecule.
- : RNA
A. MESSENGER RNA (mRNA)
B. TRANSFER RNA (tRNA)
C. RIBOSOMAL RNA (rRNA)
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